The codon-optimized DNA sequence corresponding to PMT (residues 561 to 1088) was amplified from template plasmid previously described (20) using primers designed for ligation-independent cloning. The products were cloned into the pMCSG7 expression vector previously digested with Ssp I. Plasmid was confirmed to be accurate by DNA sequencing and then transformed into BL21(DE3)(pMagic) cells. Recombinant PMT-C1C2 was purified as described above for rRRSP.

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