Briefly, overnight bacterial cultures were subcultured 1:33 and grown for 2 hours at 37°C in LB. BMDMs were infected at an MOI of 10 for 1 hour followed by gentamicin (50 μg/ml) to kill extracellular bacteria and OmpA or control buffer where indicated. Cells were collected at 2 and 16 hours after infection and lysed, and intracellular bacteria were enumerated by colony counting of serially diluted lysate.

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