PKC kinase activity was measured using the ADI-EKS-420A Assay Kit (Enzo Life Sciences, Plymouth Meeting, PA). Briefly, SlamF8+/+ or SlamF8−/− BMDMs were stimulated with 100 nM E. coli OmpA or VDAC1 for 15 min and then lysed with RIPA buffer. Protein was estimated by Bradford assay, and PKC activity in lysates was measured according to the manufacturer’s protocol.

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