Kidneys were isolated from isoflurane-anesthetized mice after perfusion with 0.9% NaCl. The kidney capsule was removed, and the tissue was cut into small pieces. The tissue was incubated at 37°C under continuous agitation (220 rpm) for 1.5 to 2 hours with a mixture of hyaluronidase (1 mg/ml) and collagenase (2.2 mg/ml) for protease digestion. The cell suspension was washed three times with phosphate-buffered saline and incubated at room temperature for 30 min with magnetic beads (Dynabeads 11047, Life Technologies) previously coated with biotinylated DBA. The cells were washed three times with isolation buffer [Dulbecco’s phosphate-buffered saline supplemented with 0.1% bovine serum albumin and 2 mM EDTA (pH 7.4)] and suspended and plated in DMEM supplemented with 10% FBS and 1% penicillin/streptomycin.

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