Antibodies for Western blot and immunohistochemistry were as follows: InsP3R1 and InsP3R3 (BD Biosciences), PC2 (a gift from Y. Cai, Yale University), VDAC (Abcam), MCU (Cell Signaling Technology, Danvers, MA), MFN2 (Cell Signaling Technology), TOM20 (Santa Cruz Biotechnology), pCREB and CREB (Cell Signaling Technology), and tubulin (Abcam). VDAC and TOM20 were used to assess mitochondrial quality and purity. β-Actin (Abcam) was used as a loading control and to assess cytoplasmic contamination of mitochondrial samples. For Western blot analyses, horseradish peroxidase–conjugated biotin-labeled secondary antibodies were used and detected with West Dura Extended Duration Substrate (Thermo Fisher Scientific).

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