Antibodies for Western blot and immunohistochemistry were as follows: InsP3R1 and InsP3R3 (BD Biosciences), PC2 (a gift from Y. Cai, Yale University), VDAC (Abcam), MCU (Cell Signaling Technology, Danvers, MA), MFN2 (Cell Signaling Technology), TOM20 (Santa Cruz Biotechnology), pCREB and CREB (Cell Signaling Technology), and tubulin (Abcam). VDAC and TOM20 were used to assess mitochondrial quality and purity. β-Actin (Abcam) was used as a loading control and to assess cytoplasmic contamination of mitochondrial samples. For Western blot analyses, horseradish peroxidase–conjugated biotin-labeled secondary antibodies were used and detected with West Dura Extended Duration Substrate (Thermo Fisher Scientific).

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.