RNA was harvested according to the manufacturer’s protocol using the E.Z.N.A. Total RNA Kit I (Omega Bio-tek; 1 to 5 μg of RNA) and was reverse transcribed with random hexamer primers using the TaqMan Reverse Transcription Reagents (Thermo Fisher Scientific). Quantitative PCR was performed using SYBR Green PCR mix (Thermo Fisher Scientific) on a StepOnePlus Real-Time PCR System (Thermo Fisher Scientific). GAPDH was used as an internal control to calculate 2−ΔCt. The following primer pairs were used: hGAPDH, 5′-TGCCATCAATGACCCCTTC-3′ and 5′-CTGGAAGATGGTGATGGGATT-3′; hIL-6, 5′-ACTCACCTCTTCAGAACGAATTG-3′ and 5′-CCATCTTTGGAAGGTTCAGGTTG-3′.

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.