RNA was harvested according to the manufacturer’s protocol using the E.Z.N.A. Total RNA Kit I (Omega Bio-tek; 1 to 5 μg of RNA) and was reverse transcribed with random hexamer primers using the TaqMan Reverse Transcription Reagents (Thermo Fisher Scientific). Quantitative PCR was performed using SYBR Green PCR mix (Thermo Fisher Scientific) on a StepOnePlus Real-Time PCR System (Thermo Fisher Scientific). GAPDH was used as an internal control to calculate 2−ΔCt. The following primer pairs were used: hGAPDH, 5′-TGCCATCAATGACCCCTTC-3′ and 5′-CTGGAAGATGGTGATGGGATT-3′; hIL-6, 5′-ACTCACCTCTTCAGAACGAATTG-3′ and 5′-CCATCTTTGGAAGGTTCAGGTTG-3′.

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