Virus-like particles were packaged in HEK293E or HEK293T cells using pCMV-MMLV-Gag-Pol or p1266 lentiviral packaging plasmids and pseudotyped with pCMV-VSV-G (gifts from P. Uchil and W. Mothes, Yale University). Packaging and transductions were performed as described previously (53). Puromycin (2 μg/ml; Sigma-Aldrich) was added 48 hours after transduction and replenished until stable pools were obtained (1 to 2 weeks).

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