Caspase-Glo 8 assays were performed according to the manufacturer’s instructions. Briefly, 1.5 × 104 cells per well (skin fibroblasts) were plated in 96-well plates. Cells were treated as described in the figure legends for indicated time periods. To measure caspase activity, 50 μl of Caspase-Glo reagent was added to each well for 15 min to ensure cell lysis, and the lysates were then transferred to a white opaque plate. Lysates were incubated for 1.5 hours with constant shaking at room temperature, which was in the linear range of reaction kinetics and did not lead to substrate depletion based on pilot experiments. Luminescence was measured using a Cytation 1 multi-mode plate reader (BioTek).

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.