Culture supernatants were recovered for LDH assay (CytoTox 96 LDH kit, Promega) and/or ELISA, and PBS containing PI (5 μg/ml; Sigma-Aldrich) was added to cells. LDH assays used untreated cells (0%) and 1% Triton X-100 in PBS (100%) to calculate the percentage of LDH released. Similarly, untreated (0%) and 0.05% Triton X-100 (100%)–treated cells were used to calculate the percentage of PI uptake as described previously (53).

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