NRCMs and fibroblasts were prepared from the ventricles of 1- to 2-day-old SD rats as described (34). H9c2 cardiac myoblasts were purchased from the American Type Culture Collection and cultured in DMEM supplemented with 10% fetal bovine serum (FBS). Plasmid DNA was transfected using Viafect (Promega) for HeLa cells or a Neon transfection system (Invitrogen) for cardiomyocytes, fibroblasts, and H9c2 cells. For siRNA knockdown, cells were transfected with each siRNA (20 nM) using Lipofectamine RNAiMAX reagent (Invitrogen) for 72 hours. Stealth siRNAs for rat FLNa (#1, RSS308835; #2, RSS308836) and rat Drp1 (#1, RSS300120; #2, RSS300122) were from Invitrogen.

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