Electrophysiological recordings were performed at room temperature with two-electrode voltage clamp or current clamp using an OC-725C Oocyte Clamp System (Warner Instruments Corp., Hamden, CT). The microelectrodes were filled with 3 M KCl (resistance, 0.5 to 2 megohms). During recording, the oocytes were superfused with ND96 solution. The Cl and HCO3-containing solutions were of 71.0 mM NaCl, 25.0 mM NaCl or NaHCO3, 2.0 mM KCl, 1.8 mM CaCl2, 1.0 mM MgCl2, and 5.0 mM Hepes-Na (pH 7.5). Solutions were constantly gassed with 5% CO2/95% O2. Current and voltage were digitized via a Digidata 1322A A/D converter (Axon Instrument, Foster City, CA) and analyzed using Clampex 8.1 system.

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