The p3xFLAG-CMV-7.1/IRBIT, pCMV-HA-IRBIT, pEGFP-C1/NBCe1-B, pEGFP-C1/NBCe2-C, and pCDNA 3.1+/NBCe1-A constructs were described previously (28). pCDNA3-Flag CaN was a gift from R. Bassel-Duby and E. Olson (University of Texas Southwestern Medical Center), pCDNA-CaMKIIα was a gift from L. Nutt (St. Jude Children’s Research Hospital, Memphis), and pCDNA3/mPP1 was a gift from E. Delpire (Vanderbilt University Medical Center, Nashville). The cDNA encoding SPAK (a gift from M. Cobb, University of Texas Southwestern Medical Center) was excised from the original vectors and transferred to pCMV-myc. Point mutations were generated by site-directed mutagenesis with a Quick light chain mutagenesis kit (Agilent), using primers to change specific regions. All constructs were verified by sequencing of the entire open reading frames.

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.