Brain sections were immunostained with antibodies against α-Syn (1:200; catalog no. 4179S, Cell Signaling Technology), NeuN (1:300; catalog no. MAB377, Millipore), pDrp-1 (1:400; catalog no. 3455S, Cell Signaling Technology), 3-NT (1:500; catalog no. ab61392, Abcam), cleaved caspase-3 (1:400; catalog no. 9661S, Cell Signaling Technology), and LC-3 (1:100; catalog no. 12741S, Cell Signaling Technology) as described earlier (36). To ensure that the homologous areas of injury were samples between animals, sections between the coordinates +1 and +1.5 from bregma were used in all cases. Analyses were performed by an investigator blinded to the study groups.

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