Statistics were calculated, and graphs were prepared using GraphPad Prism 7. When only two groups were being compared, a t test was used. When more than two groups were compared, a one-way ANOVA was performed using multiple comparisons with a Tukey correction. When data were not normal, a transformation was performed followed by analysis using a one-way ANOVA on the normalized data. *P < 0.05, **P < 0.01, and ***P < 0.001.

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