Jurkat cells were serum-starved for 6 hours before treatment for 1 hour with 80 μM dynasore (Sigma-Aldrich) and then were washed three times with PBS. Cells were then prepared for the appropriate assays. Cell surface expression of CXCR4 was measured using anti–CXCR4-PE antibody clone 4G10 and flow cytometry. Chemotaxis assays were performed as described earlier, with chemotaxis stimulated by 30 nM CXCL12.

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