Blood of wild-type, Spns2ins/+, and Spns2ins/ins adult rats were obtained from the tail vein. The blood slides were fixed in methanol for 2 to 3 min before immersing them in freshly prepared 5% Giemsa’s solution for 20 to 30 min. The slides were flushed with tap water and air dried. The counts of lymphocyte, monocyte, neutrophil, eosinophil, and basophil were obtained under an Olympus BX53 fluorescent microscope. The percentage of each cell type was calculated.

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