Western blotting was performed with antibodies from the following sources: BA1/2 (clone D24H9, #4674; Cell Signaling), CXCR4 (clone UMB2, AB124824; Abcam), CXCR4 (SAB3500383; Sigma-Aldrich), CXCR4 pSer324/325 (CP4251, ECM Biosciences), CXCR4 pSer339 (SAB4504153; Sigma-Aldrich), ERK1/2 (9102; Cell Signaling), pERK1/2 (9101; Cell Signaling), total Gαi (AB102014; Abcam), GAPDH (60004-1-Ig; Proteintech), rabbit anti-mouse (7076; Cell Signaling), and mouse anti-rabbit (7074; Cell Signaling). Confocal microscopy was performed using antibodies from the following sources: BA2 (clone C16D9, #3857; Cell Signaling), CXCR4 (clone UMB2, AB124824; Abcam), Alexa Fluor 488 goat anti-rabbit (R37116; Thermo Fisher Scientific), Alexa Fluor 555 goat anti-mouse (A21422; Thermo Fisher Scientific). For flow cytometry, staining was performed using antibodies from the following sources: PE-tagged CXCR4 4G10 monoclonal antibody (clone 4G10, sc-53534PE; Santa Cruz Biotechnology), PE-tagged CXCR4 12G5 monoclonal antibody (clone 12G5, 306506; BioLegend), PE- Mouse IgG2b, κ Isotype control antibody (402204; BioLegend), and PE-Mouse IgG2a, κ Isotype control antibody (400212; BioLegend). For immunoprecipitation, 1 μl of anti-active Gαi (26901; New East Biosciences) was used per 500 μl of sample.

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