The generation of Ryr1−/− (8), Ryr3−/− (34), Tpc1−/−Tpc2−/− (30), Orai1fl/flCd4cre (herein Orai1−/−) (35), Orai2−/−, Orai1fl/flOrai2−/−Cd4cre (8), Stim1fl/flCd4cre (Stim1−/−), Stim2fl/flCd4cre (Stim2−/−), and Stim1fl/flStim2fl/flCd4cre (Stim1−/−/Stim2−/−) (36) has been previously described. WT refers to C57BL/6 mice, and all animals were on a C57BL/6 genetic background. Sex-matched male and female mice between 6 and 13 weeks old were used. Mice were maintained under specific pathogen–free conditions in accordance with institutional guidelines for animal welfare approved by the Institutional Animal Care and Use Committees at New York University School of Medicine, University of Göttingen, University Medical Centre Hamburg-Eppendorf, and the University of Pennsylvania.

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