Fluo4-AM, Fura Red-AM, and Fura2-AM were obtained from Life Technologies. Ca2+ indicators were dissolved in DMSO, divided into aliquots, and stored at −20°C until required for use. Anti-mouse CD3 monoclonal antibody (mAb) and anti-mouse CD28 mAb were obtained from BD Biosciences.

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.