Using a series of PCRs and SOE-PCR, the nonfluorescent N- or C-terminal halves of Venus YFP (63), amplified from the 0707Up-Venus-YFP-JHMD1 gBlock, were placed between codons 2 and 3 of SGO_1180 (cytoplasmic side of TM1, creating strains 1180-TM1-V-N and 1180-TM1-V-C) or codons 61 and 62 (cytoplasmic side of TM2, creating strains 1180-TM2-V-N and 1180-TM2-V-C). Subsequently, the mated N- or C-fragments were placed in-frame between the penultimate codon and stop codon of sgo_0707 separately and in combination with the SGO_1180 N- or C-fragment strains.

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