Animals were grown at 25°C for two continuous generations in nonstarving, nonstressed conditions. One-day-old adult hermaphrodites were placed on NGM plates into the anoxia chamber for 40 hours. Animals were subsequently screened for paralysis or movement in the indicated time intervals. WormLab System (MBF Bioscience) was used to quantify displacement, moving average speed, and tracking based on the midpoint position. At least three biological replicates (10 animals per assay) were used for statistical analysis.

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