Animals were grown at 25°C for two continuous generations in nonstarving, nonstressed conditions. One-day-old adult hermaphrodites were placed on NGM plates into the anoxia chamber for 40 hours. Animals were subsequently screened for paralysis or movement in the indicated time intervals. WormLab System (MBF Bioscience) was used to quantify displacement, moving average speed, and tracking based on the midpoint position. At least three biological replicates (10 animals per assay) were used for statistical analysis.

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.