Total RNA samples were converted to Illumina sequencing libraries using Illumina’s TruSeq Stranded mRNA Sample Preparation Kit. In summary, 70 ng of mRNA was fragmented and then reverse-transcribed into cDNA. The cDNA was adenylated, then ligated to dual-indexed (barcoded) adaptors, and amplified using 15 cycles of PCR. Final library size distribution was validated using capillary electrophoresis and quantified using fluorimetry (PicoGreen). Indexed libraries were then normalized and pooled.

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.