Total RNA samples were converted to Illumina sequencing libraries using Illumina’s TruSeq Stranded mRNA Sample Preparation Kit. In summary, 70 ng of mRNA was fragmented and then reverse-transcribed into cDNA. The cDNA was adenylated, then ligated to dual-indexed (barcoded) adaptors, and amplified using 15 cycles of PCR. Final library size distribution was validated using capillary electrophoresis and quantified using fluorimetry (PicoGreen). Indexed libraries were then normalized and pooled.

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