The primary antibodies used were as follows: mouse anti-Akt [2920, Cell Signaling Technology (CST)], mouse anti-Akt–Alexa Fluor 488 (2917, CST), rabbit anti–phospho-Akt(Ser473) (4060, CST), rabbit anti–phospho-Akt(Ser473)–Alexa Fluor 647 (4075, CST), mouse anti-hemagglutinin (HA; 9658, Sigma-Aldrich), rabbit anti-EGFR (4267, CST), goat anti-EGFR (AF231, R&D Systems), mouse anti–phospho-EGFR(Tyr845) (558381, BD Biosciences), rabbit anti–phospho-EGFR(Tyr1045) (2237, CST), mouse anti–phospho-EGFR(Tyr1068) (2236, CST), goat anti-EphA2 (R&D Systems), rabbit anti–phospho-Eph(Tyr588/596) (Abcam), mouse anti-ERK1/2 (4696, CST), rabbit anti–phospho-ERK(Thr202/Tyr204) (4370, CST), mouse anti-Rab5 (610724, BD Biosciences), rabbit anti-Rab7 (9367, CST), rabbit anti–phospho-Rb(Ser807/811, CST), and mouse anti-tubulin (6074, Sigma-Aldrich). The secondary antibodies used were as follows: IRDye 680RD donkey anti-mouse (LI-COR Biosciences), IRDye 680RD donkey anti-rabbit (LI-COR Biosciences), IRDye 680RD donkey anti-goat (LI-COR Biosciences), IRDye 800CW donkey anti-mouse (LI-COR Biosciences), IRDye 800CW donkey anti-rabbit (LI-COR Biosciences), IRDye 800CW donkey anti-rabbit (LI-COR Biosciences), Alexa Fluor 405 goat anti-mouse (Life Technologies), Alexa Fluor 488 donkey anti-goat (Life Technologies), Alexa Fluor 546 donkey anti-rabbit (Life Technologies), and Alexa Fluor 647 donkey anti-rabbit (Life Technologies).

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