Animals were lysed in the Laemmli sample buffer (Bio-Rad) with reducing agent β-mercaptoethanol, followed by boiling the samples for 10 min. The worm lysates were separated by SDS-PAGE and subsequently detected by GFP goat polyclonal antibody (AF424, Fisher Scientific) with histone H3 antibody (ab1791, Abcam) as a loading control. For FLAG-tagged and HA-tagged proteins, we used FLAG mouse monoclonal antibody (F1804, Sigma-Aldrich) and HA antibody (ab9110, Abcam), respectively.

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