The Proteome Profiler or cytokine array panels (R&D Systems) were used to identify phosphorylated residues correlating to Akt-associated proteins or total chemokines or cytokines within cell populations, respectively. After the Bradford protein analysis assay to normalize total protein content, cell lysates were applied to the membranes following the manufacturer’s protocol. Western blots for total protein (for example, Akt and mTOR) were performed to confirm equal loading. Membranes were visualized by chemiluminescence (Syngene). Optical densities were determined by ImageJ software (NIH.gov) and Adobe CS5. Reference spots were used to normalize between-array membranes.

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