Cells were fixed for at least 2 hours at room temperature in 2% formaldehyde (pH 7.4), washed in 0.1 M cacodylate buffer, postfixed with 1% osmium tetroxide (OsO4)/1.5% potassium ferrocyanide (KFeCN6) for 1 hour, washed twice with water and once with maleate buffer (MB), incubated in 1% uranyl acetate in MB for 1 hour, washed twice with water, and dehydrated with grades of alcohol (10 min each, 50, 70, and 90%; 2× 10 min 100%). The samples were then put in propylene oxide for 1 hour and infiltrated overnight in a 1:1 mixture of propylene oxide and TAAB Epon (Marivac Canada Inc.). The following day, the samples were embedded in TAAB Epon and polymerized at 60°C for 48 hours. Ultrathin sections (about 60 nm) were cut on a Reichert Ultracut S microtome, picked up onto copper grids stained with lead citrate, and imaged with a JEOL 1200EX transmission electron microscope or a TecnaiG2 Spirit BioTWIN coupled to an Advanced Microscopy Techniques (AMT) 2k charge-coupled device camera.

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