WT and PARP12−/− HEK293T cells were cotransfected with the plasmids listed in the main text and, where indicated, were treated with 10 μM MG132 (Sigma-Aldrich) for 10 hours. Twenty-eight hours after transfection, cells were treated with lysis buffer [50 mM tris-HCl (pH 7.5), 150 mM NaCl, 5 mM EDTA, 1% NP-40, 1 mM phenylmethylsulfonyl fluoride (PMSF), and 1× protease inhibitor (Roche)]. The cell extracts were immunoblotted with the indicated antibodies to measure the level of the expressed proteins. Mouse anti–β-actin (ZSGB-Bio), rabbit anti-GFP (Abcam), mouse anti-poly(ADP-ribose) (GeneTex), rabbit anti-PARP12 (Sigma-Aldrich), mouse anti-ZIKV NS1 (Abcam), rabbit anti-ZIKV NS3 (GeneTex), and mouse anti-HA, anti-His, and anti-Flag tag antibodies (Sigma-Aldrich) were used for detection at the appropriate dilutions. Quantification of Western blot results was normalized to actin or correspondingly input control and pooled from three independent experiments.

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