ZIKV strain GZ01/2016 (GenBank accession number KU820898) was used at a multiplicity of infection (MOI) of 0.1 in this study, except where indicated otherwise (47). MEF cells were isolated from GD14.5 Balb/c mice. The IFNAR1−/− A549 cell line was generated as described (20). A549, BHK-21, Vero, HeLa, and HEK293T cells were purchased from American Type Culture Collection and cultured in Dulbecco’s modified Eagle’s medium (DMEM) (37°C, 5% CO2) supplemented with 10% fetal bovine serum (FBS; 100 U/ml), penicillin, and streptomycin (50 μg/ml). INO-1001 (S1132) and AG-014699 (S1098) were purchased from Selleck. MG132 (M8699) was purchased from Sigma-Aldrich, and NITD008, an adenosine nucleoside analog inhibitor that inhibits the RdRp (RNA-dependent RNA polymerase) activity of Flavivirus (48), was a gift from P.-Y. Shi (Novartis Institute for Infectious Diseases).

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