Antimicrobial peptide susceptibility assays were conducted as described (7), with modifications. Bacteria were grown in N-minimal medium with 10 μM Mg2+ (pH 7.7) or with 10 mM Mg2+ (pH 4.9) for 4 hours. Bacterial cells were then diluted 200× in phosphate-buffered saline (PBS), combined with 1:1 ratio with polymyxin B (final concentration of 5 μg/ml). After 1-hour incubation at 37°C with aeration, samples were serially diluted in PBS and plated on LB agar plates for enumeration. The percentage survival was calculated as follows: survival (%) = colony-forming units (CFU) of peptide-treated culture/CFU of no-peptide culture × 100.

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