Cultures were grown as indicated in the figure legend. Total RNA was extracted as described (51). cDNA synthesis was performed using 2 pmol of 32P-labeled 14997 primer with 10 μg of total RNA and 1 U of SuperScript II RNase H2 reverse transcriptase (Life Technologies). The extension products were analyzed by electrophoresis on a 6% acrylamide/7 M urea gel and compared with sequence ladders initiated with the same 32P-labeled primer that was used for primer extension.

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