Ba/F3–IL-6R (106) and Ba/F3-gp130 cells/ml and variants thereof were washed and starved in serum-free medium for 6 hours. Afterward, cells were stimulated with the indicated cytokines or inhibitors for 30 min [IL-3 (10 ng/ml), HIL-6 (10 ng/ml), sgp130Fc (10 μg/ml), tocilizumab (10 μg/ml), B-R3 (1 μg/ml), P6 (10 mM), and GW (3 μM)]. The cells were harvested by centrifugation at 4°C for 5 min at 1500g. Cells were suspended in Laemmli sample buffer, and pSTAT3 analysis was done by Western blotting as described.

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.