All mutagenesis PCRs were performed with the QuikChange Mutagenesis Kit (Agilent Technologies) in accordance with the manufacturer’s protocol. Gs protein (33), β-arrestin1 (minimal cysteine construct, truncated at residue 393) (27), Fab30 (27), and sortase (25) were purified as previously described. The V2Rpp-GGG phosphopeptide was synthesized and purified by the Tufts University Peptide Synthesis facility.

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