DNA sequences encoding the extracellular and transmembrane domains of FLAG-tagged mouse CD300b (LMIR5) fused with the intracellular domain of human CD3ζ (N. Matsumoto, University of Tokyo) were inserted into pMXs–internal ribosomal entry site–puror (pMXs-IP) to generate pMXs-FLAG-CD300b-CD3ζ-IP (27).

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