Statistical and graphical analyses were performed using GraphPad Prism software unless otherwise indicated. Data were analyzed by two-tailed t test or one-way ANOVA with Fisher’s LSD post hoc analysis. Curve fitting of FRAP data was done with a two-phase association nonlinear function using the least-squares fit. For single-cell analysis (dcFRAP), the number of the individual ROIs obtained from several cells in at least three independent experiments is provided in the figure legends. Data from individual ROIs are summarized for data presentation, providing the sum of independent observations. Significance levels are given as: *P < 0.05, **P < 0.01, ***P < 0.001. All data points throughout the manuscript represent the means ± SEM.

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