Statistical and graphical analyses were performed using GraphPad Prism software unless otherwise indicated. Data were analyzed by two-tailed t test or one-way ANOVA with Fisher’s LSD post hoc analysis. Curve fitting of FRAP data was done with a two-phase association nonlinear function using the least-squares fit. For single-cell analysis (dcFRAP), the number of the individual ROIs obtained from several cells in at least three independent experiments is provided in the figure legends. Data from individual ROIs are summarized for data presentation, providing the sum of independent observations. Significance levels are given as: *P < 0.05, **P < 0.01, ***P < 0.001. All data points throughout the manuscript represent the means ± SEM.

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.