HPAF-II and PANC-1 cells were seeded at 1000 to 2000 cells per well in 96-well plates. The next day, cells were treated with the PORCN inhibitor C59, the Gαq inhibitor FR900359, or vehicle in technical triplicate at the indicated concentrations. Medium containing inhibitor or vehicle was replaced every 3 days. Cell viability was measured using Alamar Blue (Thermo Fisher Scientific, catalog no. DAL1025) approximately 6 to 7 days after seeding following the manufacturer’s instructions. Fluorescence was measured at 560-nm excitation and 590-nm emission with a SPECTRAmax GEMINI Microplate Spectrofluorometer plate reader (Molecular Devices).

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.