CLB-BAR cells were seeded on six-well plates precoated with 0.4% solution of type I bovine collagen solution (Advanced BioMatrix, lot no. 7434). Cells were treated with lorlatinib (30 nM) for 30 min or not treated before lysis with radioimmunoprecipitation assay buffer. Lysates were treated identically with one sample treated with 1500 U of λ protein phosphatase (P0753S, New England BioLabs Inc.). 4× Laemmli buffer was added to the lysates before Western blot analysis.

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