FlAsH labeling was done as previously described (25, 29). Briefly, cells were washed once with Hank’s balanced salt solution (HBSS) containing glucose (1.8 g/liter) and then incubated at 37°C for 1 hour with HBSS supplemented with 500 nM FlAsH and 12.5 μM 1,2-ethanedithiol (EDT). After that, cells were rinsed twice with HBSS, incubated for 10 min with HBSS containing 250 μM EDT, and washed again twice with HBSS. Last, cells were maintained in DMEM before measurements.

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