Rats were anesthetized with sodium pentobarbital (50 mg/kg, i.p.) and perfused through the ascending aorta with 4% paraformaldehyde. The L4 to L6 DRGs were removed, postfixed in the same fixative for 3 hours, and transferred to 30% sucrose overnight. Cryostat sections (8 μm) were cut and processed for immunohistochemistry using primary antibodies. After incubation overnight at 4°C, the sections were incubated with secondary antibodies for 1 hour at room temperature. Images were captured using an SIM super-resolution microscope system with 78-nm resolution in the x and y axes and 300-nm resolution in the z axis (Nikon).

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