Intrathecal injection of 2-BP (10 μl; Sigma-Aldrich) was performed according to our previously described method (25). Briefly, a polyethylene-10 catheter (Smiths) was inserted into the rat’s subarachnoid space through L5 to L6 spinal cord segmental intervertebral space, and the tip of the catheter was located at the L5 spinal segmental level. 2-BP was intrathecally injected 30 min before the first dose of oxaliplatin or L5-VRT surgery once per day.

The 50% withdrawal threshold was assessed using von Frey hairs (Ugo Basile) as described previously (43). Briefly, each animal was loosely restrained in a plastic box on a metal mesh and allowed to acclimate for at least 15 min per day for three consecutive days. On the first testing day, the animals were reintroduced to the testing environment and allowed to accommodate, and then series of von Frey filaments with different bending force were presented alternately from the underneath to the mid-plantar surface of each hind paw. In the absence of a paw withdrawal response to the initially selected hair, a stronger stimulus was presented; in the event of paw withdrawal, the next weaker stimulus was chosen. Optimal threshold calculation by this method requires six responses in the immediate vicinity of the 50% threshold. A nociceptive response was defined as a brisk paw withdrawal or flinching of the paw after von Frey filament application. All the experiments were performed by investigators that were blinded to the treatments/conditions.

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.