Retroviral expression vectors pLXSN expressing a chimeric receptor (CDMer) generated from the extracellular and transmembrane domains of human CD8 (amino acids 1 to 209) and the intracellular region (amino acids 521 to 994) of WT MerTK, K614M KD MerTK, Y825F MerTK, Y867F MerTK, or Y924F MerTK were generated and used to transfect HEK 293T cells as described previously (49, 50).

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