To be considered as a target for S-nitrosylation, proteins detected from MS had to be enriched ≥2-fold in CysNO samples versus control in three experiments and not enriched ≥2-fold in any control. Sodium ascorbate is required as a reducing agent for S-nitrosothiols; therefore, samples in which sodium ascorbate were omitted were used as negative controls (see table S2).

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