The cytomegalovirus (CMV) myc-CREB plasmid was created by B. Lonze (Ginty Lab). msMBD3, ratRBBP4, and ratRBBP7 were subcloned into the CMV-myc expression vector. Mutagenesis to create RBBP7, RBBP4 and MBD3 cystine-to-serine mutants, MBD3 lysine-to-alanine mutants, and mycRBBP7WT and mycRBBP7C166S siRNA-resistant plasmids was carried out using a QuikChange multisite-directed mutagenesis kit (Agilent). HA-tagged ratRBBP7 and HA-tagged msHDAC2 were each cloned into AdEasy pShuttle-CMV vectors, and cysteine and lysine mutants were created using QuikChange multisite-directed mutagenesis kit (Agilent). Flag-mCHD4, hCHD5, and hCHD3 were generated as previously described (21). siRNAs were generated by Invitrogen. The following sequences were used: siRBBP7, 5′-CCACAUAAUGAAACUAUUCUGGCUU-3′ (forward) and 5′-AAGCCAGAAUAGUUUCAUUAUGUGG-3′ (reverse); siRBBP4, 5′-AAAUCUUUCCCUUCAGGCCUGGUCA-3′ (forward) and 5′-UGACCAGGCCUGAAG-GGAAA-GAUUU-3′ (reverse).

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.