Standard silver staining protocol was followed. Briefly, the polyacrylamide gel was soaked in 50% methanol two times for 15 min and then in 5% methanol for 10 min before rinsing in water. Gel was then soaked in 10 μM DTT for 20 min and then in 0.1% silver nitrate for 20 min. After three washes with water, gel was washed two times in developer solution (500 ml of ~285 mM sodium carbonate solution plus 250 μl of 37% formaldehyde) and soaked until bands became visible. The reaction was stopped with addition of citric acid, and gel was washed in water before imaging.

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