Postnuclear lysates were incubated with prewashed Strep-Tactin Sepharose beads (IBA GmbH) for 1.5 hours at 4°C on a rotary wheel. Beads were washed two times with 1 ml of lysis buffer complemented with detergent and phosphatase inhibitors and three times with 1 ml of lysis buffer in the absence of detergent and protease-phosphatase inhibitors. Proteins were eluted from the Strep-Tactin Sepharose beads with 2.5 mM d-biotin–containing buffer. Samples were analyzed by nano-LC coupled to either an LTQ Orbitrap Velos mass spectrometer (Thermo Fisher Scientific) (for samples of biological replicates 1 to 3) or a Q-Exactive mass spectrometer (Thermo Fisher Scientific) (for samples of biological replicate 4).

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