Preparation of cortical neurons
Cell culture and transfections
Biotin-switch technique
S-nitrosothiol resin-assisted capture
Silver staining
Coimmunoprecipitation
Western blotting
Dendritogenesis assay
Plasmids
GO analysis
Mass spectrometry
SNO-site identification
Protein identification from neuronal extracts
NanoLC-MS/MS analysis of neuronal extracts
Analysis of neuronal extract data
Hit criteria for SNO-Ps
Motif-X analysis
Statistical analysis
All animal experiments were approved by the University College London Animal Welfare and Ethical Review Body and carried out in accordance to appropriate UK Home Office licenses. Timed pregnant Sprague-Dawley rats and C57BL/6J WT mice were used to prepare embryonic cortical neurons.