rmTNF-a (50 ng/ml; Peprotech) was used unless otherwise indicated. z-VAD-fmk (Enzo) was used at 20 μM. Low–molecular weight poly(I:C) (50 μg/ml; Invivogen) was used. AP21967 (A/C heterodimerizer; Clontech) was used at the indicated concentrations. RIPK3 inhibitors GSK′842 (Aobious) and GSK′872 (Millipore) were used at the indicated concentrations.

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