The following proteins were used (details, catalog numbers, and suppliers of proteins are listed in table S5): recombinant human TGF-β1; purified human TSP1; recombinant human proLOX; rProCOL1A1; denatured bovine collagen I; pepsin-digested collagens I, II, and III; native collagen I fibrils; and CPI and CPIII homotrimers (4, 72). Collagens were dissolved overnight in 0.5 M HAc to final concentrations of 1 or 5 mg/ml. Collagen Toolkit II and derivative peptides were synthesized as C-terminal amides on TentaGel R RAM resin using Fmoc (9-fluorenyl methoxycarbonyl)/tBu (tert-butyl) method (44) and using CEM Liberty or Liberty Blue automated microwave peptide synthesizers. Each guest sequence (27 residues for Toolkits or shorter sequences containing KGHR and its derivatives, as indicated) is placed between GPC(GPP)5– and –(GPP)5GPC host sequences to stabilize the triple-helical conformation. GPP10, a negative control peptide (GPC-[GPP]10GPC-NH2), represents the combined host sequence. All peptides were dissolved in 10 mM HAc at a final concentration of 5 mg/ml.

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