GSH was measured using Glutathione Assay Kit (703002, Cayman Chemicals) according to the manufacturer’s instructions. Briefly, astrocytes were harvested in MES buffer after 6-hour incubation with DEM and sonicated to disrupt cellular membrane. Supernatants were incubated with 10% meta-phosphoric acid (MPA) (Sigma) at room temperature for deproteination. Total GSH was detected by measuring the product of glutathionylated 5,5′-dithiobis(2-nitrobenzoic acid) (DTNB) at 415 nm with a kinetic method on an ultraviolet spectrophotometer. GSH amount was normalized by the protein amount per each sample.

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