Body weight, occult or gross blood lost per rectum, and stool consistency were assessed as briefly described (91). The baseline clinical score was determined on day 0. Scoring went as follows: No weight loss was scored as 0, weight loss of 1 to 5% as 1, 5 to 10% as 2, 10 to 20% as 3, and more than 20% as 4. For stool consistency, a score of 0 was assigned for well-formed pellets, 2 points for semiformed stools, and 4 points for liquid stools. For bleeding, a score of 0 points was assigned for no blood, 2 points for positive occult blood test (Sure-Vue Fecal Occult Blood Test, Fisher HealthCare), and 4 points for gross bleeding. For CD4+CD45RBhigh T cell transfer weight loss, bleeding, and diarrhea scores were shown. For experimental colitis induced with DSS, all scores were added together and divided by three, resulting in a total clinical score.

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.