Mice at P21-28 were anesthetized and placed in the mouse stereotaxic frame [World Precision Instrument (WPI)] to secure the cranium. Then, the mice were injected with 250 to 500 nl of AAV (2.0 × 1010 genomic copies (GC)/μl) at the rate of 100 to 200 nl/min into the mPFC bilaterally using a NanoFil syringe (WPI) with a 35G beveled needle. The following stereotactic coordinate was used for injection: anteroposterior (AP), +1.5 mm; mediolateral (ML), ±0.2 mm; and dorsoventral (DV), −1.8 mm from the bregma. Three to four weeks later, LPS (5 mg/kg; Sigma, O55:B5) was injected intraperitoneally, and the brains were harvested 48 hours later.

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